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dithiothreitol  (AMS Biotechnology)


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    Structured Review

    AMS Biotechnology dithiothreitol
    Dithiothreitol, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/39759%2E02/Dithiothreitol/custom%4039759%2E02%4037208684
    Average 96 stars, based on 87 article reviews
    dithiothreitol - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: The effects of microRNAs on human neural stem cell differentiation in two- and three-dimensional cultures.
    Article Snippet: .. Western blot analysis HNSC cell monolayers were lysed with 1× SDS sample buffer and dithiothreitol (DTT) reducing agent (AMS Biotechnology), and loaded onto a PAGEgel (Invitrogen). ..

    Article Title: The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria.
    Article Snippet: ROS production in leaf disks (two disks per plant) was analyzed by luminol reaction (100 lM luminol, 10 lg ml 1 horseradish peroxidase (HRP)) using a microplate luminescence reader (Mithras LB 940, Berthold Technologies, GmbH & Co. KG, Bad Wildbad, Germany). .. Total proteins were extracted by grinding two 7.5-mm-diameter leaf disks in 100 ll of 39 loading buffer containing 50 mM dithiothreitol (DTT), and the presence of VvFLS2-GFP was detected by Western blot using rabbit anti-GFP antibodies (AMS Biotechnology, Abingdon, UK) and goat anti-rabbit-HRP secondary antibodies (SigmaAldrich). ..

    Centrifugation:

    Article Title: Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number
    Article Snippet: .. OD 660 ∼0.7) yeast cultures grown in YPG in 5-liter flasks, under ∼150 rpm agitation at 30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $^\circ$\end{document} C. Cells were harvested at 3993 × g for 5 min and washed twice with 50 ml of H 2 O with a 2 min centrifugation at 3146 × g . Cells were incubated for 10 min at 75 rpm agitation and 30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $^\circ$\end{document} C in 2 ml/g wet weight of alkaline solution [100 mM Tris and 10 mM dithiothreitol (DTT)] and pelleted as also in the following washing step at 1861 × g for 5 min. After washing with 6.7 ml/g wet weight in spheroplast buffer (20 mM KH 2 PO 4 pH 7.4, 1.2 M sorbitol), cells were gently resuspended in the same buffer containing 6/6.6 mg/ml Zymolyase (R) 20T (Amsbio 120491-1) to digest cell walls for 30 min at 30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $^\circ$\end{document} C and 75 rpm agitation. ..

    Incubation:

    Article Title: Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number
    Article Snippet: .. OD 660 ∼0.7) yeast cultures grown in YPG in 5-liter flasks, under ∼150 rpm agitation at 30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $^\circ$\end{document} C. Cells were harvested at 3993 × g for 5 min and washed twice with 50 ml of H 2 O with a 2 min centrifugation at 3146 × g . Cells were incubated for 10 min at 75 rpm agitation and 30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $^\circ$\end{document} C in 2 ml/g wet weight of alkaline solution [100 mM Tris and 10 mM dithiothreitol (DTT)] and pelleted as also in the following washing step at 1861 × g for 5 min. After washing with 6.7 ml/g wet weight in spheroplast buffer (20 mM KH 2 PO 4 pH 7.4, 1.2 M sorbitol), cells were gently resuspended in the same buffer containing 6/6.6 mg/ml Zymolyase (R) 20T (Amsbio 120491-1) to digest cell walls for 30 min at 30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $^\circ$\end{document} C and 75 rpm agitation. ..

    other:

    Article Title: Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number.
    Article Snippet: Membranes were washed in TBS for at least 30 min with three buffer exchanges at 25 ◦C, before chemiluminescent signals were detected with a Vilber Fusion FX imaging system, after a 1:1 mixture of HRP substrate on 17 January 2026 solution 1 [100 mM Tris–HCl pH 8.5, 0.44 mg/ml Luminol (from 100 × DMSO stock), 0.066 mg/ml p -coumaric acid (from 227 × DMSO stock)] and solution 2 (100 mM Tris– HCl pH 8.5, 0.18 μl/ml hydrogenperoxide) was applied to the blots

    Lysis:

    Article Title: Mutations that prevent methylation of cohesin render sensitivity to DNA damage in S. pombe
    Article Snippet: .. 6 ml of exponentially growing cells were harvested and resuspended in 1 ml of spheroplasting solution (0.65 M KCl with 10 mM dithiothreitol) including 20 μl zymolyase (10 mg/ml; AMS Biotechnology), 20 μl glucuronidase (0.4%; Sigma-Aldrich), 60 μl lysing enzymes (250 mg/ml; L1412, Sigma-Aldrich) and digested at 30°C until lysis of spheroplasts was observed in 1% sodium sarcosyl. ..



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